Volume 27, Number 2, March 1999Capillary electrophoresis
|Page(s)||166 - 169|
Differential display analysis of gene expression accompanied by neurite outgrowth of human neuroblastoma Cell IMR32 using non-gel molecular sieving capillary electrophoresisN. Ishioka1, Y. Kurosu2, 3, A. Kuhara4, T. Kogure4, 5, Y. Ueno4, 6, M. Saito4, 6, K. Watabe7 and I. Nagaoka2
1 Space Utilization Systems, Tsukuba Space Center, National Space Development Agency of Japan (NASDA), 2-1-1, Sengen, Tsukuba-shi, Ibaraki 305-8505, Japan
2 The department of Biochemistry, Juntendo University, School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
3 JASCO Technical Research Laboratories Corporation, 2097-2, Ishikawa-cho, Hachioji-shi, Tokyo 192-0032, Japan
4 Division of Molecular Cell Biology, Institute of DNA Medicine, The Jikei university School of Medicine, 3-25-8, Nishi-Shinbashi, Minato-ku, Tokyo 105-0003, Japan
5 Department of Neuro Surgery, The Jikei university School of Medicine, 3-25-8, Nishi-Shinbashi, Minato-ku, Tokyo 105-0003, Japan
6 Department of Orthopedic Surgery, The Jikei university School of Medicine, 3-25-8, Nishi-Shinbashi, Minato-ku, Tokyo 105-0003, Japan
7 Department of Neuropathology, Tokyo Metropolitan Institute for Neuroscience, 2-6, Musashidai, Fuchu-shi, Tokyo 183-0042, Japan
The induced proliferative response of the human derived neuroblastoma cell (IMR32), with the cell proliferation mediating factor, is much milder compared to other cell strains, and develops cellular clusters which are characterized by numerous extensions of dendrites. Presuming this phenomenon to be one of the induced neuronal differentiation due to genetic alterations, we preliminary studied to elucidate the changes in gene expression of the IMR32 by mRNA differential display analysis, monitoring polymerase chain reaction (PCR) products by non-gel molecular sieving capillary electrophoresis in linear polyacrylamide solution. The CE fingerprints revealed a number of peaks with differential expression patterns.
Key words: Differential display / RT-PCR / neuroblastoma / cell proliferation / capillary electrophoresis / molecular sieving / linear polyacrylamide.
© EDP Sciences, Wiley-VCH 1999