Free Access
Volume 27, Number 2, March 1999
Capillary electrophoresis
Page(s) 163 - 165
Section Original articles
Analusis 27, 163-165 (1999)
DOI: 10.1051/analusis:1999162

Quantification of phenolphthalein in cosmetic products

L. Gagliardi1, D. De Orsi1, G. Multari1, G. Cavazzutti1 and D. Tonelli2

1  Laboratorio di Chimica del Farmaco-Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, Italy
2  Dipartimento di Chimica Fisica ed Inorganica, Università di Bologna, Viale Risorgimento 4, 40136 Bologna, Italy

A simple method for the quantification of phenolphthalein in cosmetic formulations is described. The sample, acidified with acetic acid, is extracted with methanol and analysed by reversed-phase HPLC on a column packed with 5 $\mu$m SelectB RP-8. The initial mobile phase is acetonitrile-aqueous 0.1 M acetic acid (10:90), then a linear gradient up to 90% acetonitrile is applied in 30 min. The limit of determination is 1 ng injected when detection is performed at 230 nm.

Key words: Phenolphthalein / high-performance liquid chromatography / cosmetics.

© EDP Sciences, Wiley-VCH 1999