Free Access
| Issue |
Analusis
Volume 27, Number 2, March 1999
Capillary electrophoresis
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|---|---|---|
| Page(s) | 163 - 165 | |
| Section | Original articles | |
| DOI | https://doi.org/10.1051/analusis:1999162 | |
Analusis 27, 163-165 (1999)
DOI: 10.1051/analusis:1999162
1 Laboratorio di Chimica del Farmaco-Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, Italy
2 Dipartimento di Chimica Fisica ed Inorganica, Università di Bologna, Viale Risorgimento 4, 40136 Bologna, Italy
Key words: Phenolphthalein / high-performance liquid chromatography / cosmetics.
© EDP Sciences, Wiley-VCH 1999
DOI: 10.1051/analusis:1999162
Quantification of phenolphthalein in cosmetic products
L. Gagliardi1, D. De Orsi1, G. Multari1, G. Cavazzutti1 and D. Tonelli21 Laboratorio di Chimica del Farmaco-Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, Italy
2 Dipartimento di Chimica Fisica ed Inorganica, Università di Bologna, Viale Risorgimento 4, 40136 Bologna, Italy
Abstract
A simple method for the quantification of phenolphthalein in cosmetic
formulations is described. The sample, acidified with acetic acid,
is extracted with methanol and analysed by reversed-phase HPLC on a
column packed with 5 
m SelectB RP-8. The initial mobile phase is
acetonitrile-aqueous 0.1 M acetic acid (10:90), then a linear gradient
up to 90% acetonitrile is applied in 30 min. The limit of determination
is 1 ng injected when detection is performed at 230 nm.
Key words: Phenolphthalein / high-performance liquid chromatography / cosmetics.
© EDP Sciences, Wiley-VCH 1999