Volume 27, Number 2, March 1999Capillary electrophoresis
|Page(s)||170 - 173|
A FIA procedure for enzymatic determination of high glucose concentrations. Application to parenteral solutions analysisE. Barrado1, M.H. Vela1, R.A.S. Lapa2 and J.L.F.C. Lima2
1 Departamento de Química Analítica, Facultad de Ciencias, Prado de la Magdalena s/n, Universidad de Valladolid, 47005 Valladolid, España
2 CEQUP/Departamento de Química-Física, Faculdade de Farmácia, Rua Anibal Cunha 164, Universidade de Porto, 4050 Porto, Portugal
A flow injection system for the determination of high levels of glucose in parenteral solutions based on amperometric detection of hydrogen peroxide produced in the glucose oxidase reaction was developed. The determinations were made without any sample pre-treatment. The flow injection manifold includes two dialysis units to perform dilution of the samples and to maintain the enzyme in a closed re-circulation loop. Due to the hydrodynamic characteristics of the analytical system, the variables were optimized using a parameter design of Taguchi. Under optimal conditions, a linear response for glucose concentrations, between 0.1 and 1.0 M was achieved. A good reproducibility (r.s.d. ) and a sampling rate of 30 determinations/hour were observed. The results obtained are in a good agreement with those obtained using the spectrophotometric enzymatic method.
Key words: Flow injection analysis / amperometry / glucose / parenteral solutions / Taguchi parameter design / glucose oxidase reaction.
© EDP Sciences, Wiley-VCH 1999