Issue |
Analusis
Volume 26, Number 3, April 1998
Analyse des corps gras
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Page(s) | 107 - 115 | |
Section | Original articles | |
DOI | https://doi.org/10.1051/analusis:1998119 |
DOI: 10.1051/analusis:1998119
Optimization of the indirect fluorimetric detection for the simultaneous analysis of inorganic anions and polycharged organic anions by capillary electrophoresis
P.L. Desbène, C. Morin and A. DesbèneLaboratoire d'Analyse des Systèmes Organiques Complexes, I.R.C.O.F. et I.F.R. n° 23, Université de Rouen, 43 rue Saint-Germain, 27000 Evreux, France
Abstract
Until now, fluorescein sodium salt has been used to generate the
background signal for indirect fluorimetric detection of inorganic
cations or mono-charged organic anions separated by Capillary
Zone Electrophoresis (CZE). In this paper, we extend the use of
fluorescein sodium salt to the simultaneous CZE analysis of inorganic
anions (Cl-, NO-2, NO-3, SO2-4, F-) and polycharged
organic anions (oxalate and citrate). The electroosmotic flow was reversed
by using tetradecyltrimethylammonium bromide (TTAB) or hexadimethrine.
The specific problems linked to the simultaneous utilization of fluorescein
sodium salt and a positively charged capillary were studied and resolved by
reducing the fluorescein/TTAB interactions and by optimizing the fluorophore
concentration in the electrolyte. The study of reproducibility and the
quantitative approach led to very good results. In such conditions the
quantification of Cl-, NO-3 and SO2-4, in different mineral
and spring waters, has been successfully realized. In addition, the
detection thresholds reached the range of one hundred to a few hundred ppb.
Key words: Capillary zone electrophoresis / indirect fluorimetric detection / fluorescein sodium salt / inorganic anions / polycharged organic anions.
© EDP Sciences, Wiley-VCH 1998