Volume 28, Number 9, November 2000Endocrine disruptors
|Page(s)||843 - 846|
Evidence of butyl benzyl phtalate induced modifications in a model system developed in vitroM.G. Sabbieti1, L. Marchetti1, R. Curini2, G. Menghi1, A. Roda3, M.V. Russo4, C. Nugnes2 and S. Materazzi2
1 Dep. of Comparative Morphological and Biochemical Sciences, University of Camerino, Italy
2 Dep. of Chemistry, University "La Sapienza", p. le A. Moro 5, 00185 Roma, Italy
3 Dep. of Chemistry, University of Bologna, Italy
4 DISTAAM, University of Molise, Campobasso, Italy
(Received August 1, 2000; revised September 26, 2000; accepted October 5, 2000.)
The phtalate induced modifications on osteoblastic cells have been monitored in a model system in vitro: preliminary encouraging results, independently obtained by calorimetry (DSC), confocal laser scanning microscopy (CLSM) and coupled gas chromatography-mass spectrometry (GC-MS), show the possibility to relate kinetic data and immunofluorescence images, that are very useful to show the entry and the localization of phtalate into the cells. The calorimetric data were obtained by a cellular living system growth and reacting into the aluminium pans.
Key words: Butyl benzyl phtalate -- rat osteoblasts -- CLSM -- DSC -- GC-MS.
© EDP Sciences, Wiley-VCH 2000