Analusis 28, 819-824 (2000)
DOI: 10.1051/analusis:2000149

Determination of glyphosate herbicide and (aminomethyl)phosphonic acid (AMPA) in water by liquid chromatography and fluorescence detection. Part II: Direct determination using pre-column derivatization with NBD-Cl

R. Colin^{1, 2}, E. Le Fur¹, C. Charrêteur¹, C. Dufau² and J.-J. Péron<sup>2

1</sup>  Centre de Génie Industriel, Guidel-Plages, 56520 Guidel, France
²  Laboratoire de Biologie et Chimie Moléculaires, Université de Bretagne Sud, rue Saint-Maudé, 56325 Lorient Cedex, France

(Received July 6, 2000; revised September 12, 2000; accepted September 19, 2000.)

Abstract
An analytical method was developed for the determination of glyphosate and AMPA in water. This method involves derivatization in situ with NBD-Cl reagent at 60 °C. The quantification is performed using liquid chromatography with fluorescence detection after a separation on an amino column. The limit of quantification was estimated at 1 g/L level for glyphosate and at 0.1 g/L level for AMPA.

Key words: glyphosate -- AMPA -- analysis -- fluorescence -- NBD-Cl.


© EDP Sciences, Wiley-VCH 2000