Volume 28, Number 9, November 2000Endocrine disruptors
|Page(s)||819 - 824|
Determination of glyphosate herbicide and (aminomethyl)phosphonic acid (AMPA) in water by liquid chromatography and fluorescence detection. Part II: Direct determination using pre-column derivatization with NBD-ClR. Colin1, 2, E. Le Fur1, C. Charrêteur1, C. Dufau2 and J.-J. Péron2
1 Centre de Génie Industriel, Guidel-Plages, 56520 Guidel, France
2 Laboratoire de Biologie et Chimie Moléculaires, Université de Bretagne Sud, rue Saint-Maudé, 56325 Lorient Cedex, France
(Received July 6, 2000; revised September 12, 2000; accepted September 19, 2000.)
An analytical method was developed for the determination of glyphosate and AMPA in water. This method involves derivatization in situ with NBD-Cl reagent at 60 °C. The quantification is performed using liquid chromatography with fluorescence detection after a separation on an amino column. The limit of quantification was estimated at 1 g/L level for glyphosate and at 0.1 g/L level for AMPA.
Key words: glyphosate -- AMPA -- analysis -- fluorescence -- NBD-Cl.
© EDP Sciences, Wiley-VCH 2000