Free Access
Volume 28, Number 9, November 2000
Endocrine disruptors
Page(s) 819 - 824
Section Original articles
Analusis 28, 819-824 (2000)
DOI: 10.1051/analusis:2000149

Determination of glyphosate herbicide and (aminomethyl)phosphonic acid (AMPA) in water by liquid chromatography and fluorescence detection. Part II: Direct determination using pre-column derivatization with NBD-Cl

R. Colin1, 2, E. Le Fur1, C. Charrêteur1, C. Dufau2 and J.-J. Péron2

1  Centre de Génie Industriel, Guidel-Plages, 56520 Guidel, France
2  Laboratoire de Biologie et Chimie Moléculaires, Université de Bretagne Sud, rue Saint-Maudé, 56325 Lorient Cedex, France

(Received July 6, 2000; revised September 12, 2000; accepted September 19, 2000.)

An analytical method was developed for the determination of glyphosate and AMPA in water. This method involves derivatization in situ with NBD-Cl reagent at 60 °C. The quantification is performed using liquid chromatography with fluorescence detection after a separation on an amino column. The limit of quantification was estimated at 1 $\mu$g/L level for glyphosate and at 0.1 $\mu$g/L level for AMPA.

Key words: glyphosate -- AMPA -- analysis -- fluorescence -- NBD-Cl.

© EDP Sciences, Wiley-VCH 2000