| Issue |
Analusis
Volume 28, Number 9, November 2000
Endocrine disruptors
|
|
|---|---|---|
| Page(s) | 813 - 818 | |
| Section | Original articles | |
| DOI | https://doi.org/10.1051/analusis:2000148 | |
DOI: 10.1051/analusis:2000148
Determination of glyphosate herbicide
and aminomethylphosphonic acid in natural waters
by liquid chromatography using pre-column
fluorogenic labeling. Part I: Direct determination
at the 0.1 
g/L level using FMOC
E. Le Fur1, R. Colin1, 2, C. Charrêteur1, C. Dufau2 and J.-J. Péron2 1 Centre de Génie Industriel, Guidel-plages, F-56520 Guidel, France
2 Laboratoire de Biologie et Chimie Moléculaires, Université de Bretagne Sud, rue Saint Maudé, F-56325 Lorient, France
(Received July 6, 2000; revised September 12, 2000; accepted September 19, 2000.)
Abstract
An analytical method has been developed for the determination of glyphosate herbicide
and its metabolite aminomethylphosphonic acid (AMPA) in natural waters to a level
of 0.10 g/L with a good linearity in the range 0.10-2.00 g/L and coefficients of
variation under 20% for each spiked level. The procedure involves a precolumn
derivatization step with
9-fluorenylmethylchloroformate (FMOC-Cl) yielding highly fluorescent derivatives
of the analytes which then can be determined by HPLC with fluorescence detection.
The method is used for analysis of natural (surface and ground) and treated waters.
Matrice effects have been pointed out, particularly the effects of hard ionic
content and the effects of chlorine residues into treated waters.
Key words: Glyphosate -- AMPA -- HPLC -- fluorescence -- FMOC.
© EDP Sciences, Wiley-VCH 2000