Analusis 28, 132-135 (2000)
DOI: 10.1051/analusis:2000105

A comparative analysis of retention values obtained on fused silica capillary columns from various manufacturers

V.G. Berezkin, A.A. Korolev and I.V. Malyukova

Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninsky pr., 29, 117912 Moscow, Russia

(Received July 21, 1999; revised February 10, 2000; accepted February 22, 2000.)

Abstract
A comparative analysis of retention characteristics obtained on fused silica capillary columns available from various manufacturers is performed. The retention indexes for organic compounds of different chemical classes (octanol-1, 2,6-dimethylphenol, 2,6-dimethylaniline, naphthalene, methylpelargonate) are measured on the columns. The maximum variation in retention indexes is 1,63 i.u. on the columns coated with nonpolar stationary liquid phase (polydimethylsiloxane) and 16.26 i.u. on those coated with polar that (polyethylene glycol). The average maximum variation in the retention indexes obtained on the polyethylene glycol columns is more than that on the polydimethylsiloxane columns by a factor of 5. The established variation in columns selectivity from various manufacturers must be taken into account both in routine chromatography, in studies of physicochemical characteristics and when compiling of data bases.

Key words: Capillary chromatography -- capillary column -- reproducibility of retention values -- comparison of capillary columns from various manufacturers.


© EDP Sciences, Wiley-VCH 2000