Free Access
Volume 27, Number 10, December 1999
Page(s) 835 - 838
Section Original articles
Analusis 27, 835-838 (1999)
DOI: 10.1051/analusis:1999101

Fluorimetric nitrite analysis using 2,3-diaminonaphthalene: an improvement of the method

M.-C. Carré, B. Mahieuxe, J.-C. André and M.-L. Viriot

DCPR-GRAPP - UMR 7630 CNRS-INPL, Groupe ENSIC, 1, rue Grandville, 54000 Nancy, France

(Received June 8, 1999; revised October 1, 1999; accepted October 5, 1999.)

One of the fluorimetric methods for nitrite determination is based on the reaction of nitrite ions with 2,3-diaminonaphthalene (DAN) to form fluorescent 1-[H]-naphthotriazole. The procedure is sensitive, simple and rapid. However, the DAN reagent is carcinogenic and its use in a large excess for the reaction might be a drawback for an extensive application. In particular to know if a stoechiometric quantity of the reagent might be sufficient to run the reaction, the method was re-examined. In fact, using the latter condition, it was still possible to get nitrite determination in the range 0 to 50 $\mu$g NO2-.L-1, with a rather smaller analysis time.

Key words: Nitrite / Fluorescence / 2,3-diaminonaphthalene.

© EDP Sciences, Wiley-VCH 1999