Free Access
Volume 27, Number 1, January/February 1999
Importance de la lipophilie en modélisation moléculaire
Page(s) 69 - 74
Section Original articles
Analusis 27, 69-74 (1999)
DOI: 10.1051/analusis:1999109

Quantitative analysis of pungent principles of pepper oleoresins: Comparative study of three analytical methods

I. Noyer1, B. Fayet1, I. Pouliquen-Sonaglia2, M. Guerere1 and J. Lesgard2

1  Laboratoire Interrégional de Marseille, Direction Générale de la Concurrence Consommation et Répression des Fraudes, 146 traverse Charles Susini, 13388 Marseille Cedex 13, France
2  Faculté des Sciences et Techniques de Saint-Jérôme, Laboratoire de Chimie de Produits Naturels, avenue Escadrille Normandie Niémen, 13397 Marseille Cedex 20, France

For the determination of pungent compounds in pepper oleoresins, three analytical methods are described and compared to determine which of them is the most available for routine analysis and for informations about relative amount of all alkaloids. These methods are the UV spectrophotometry, the high-performance liquid-chromatography and the gas-chromatography. These analysis were carried out with 26 pepper oleoresins samples. The three methods were compared with two statistical tests, both defined in a French Norm from AFNOR. It seems that HPLC and GC methods, considering only the peak of piperine, are comparable. In the same way, HPLC and UV-spectrophotometry methods give similar results of total amount of alkaloids.

Key words: Oleoresin / piperine / UV spectrophotometry / high-performance liquid-chromatography (HPLC) / gas chromatography (GC)

© EDP Sciences, Wiley-VCH 1999