Free Access
Volume 27, Number 1, January/February 1999
Importance de la lipophilie en modélisation moléculaire
Page(s) 65 - 68
Section Original articles
Analusis 27, 65-68 (1999)
DOI: 10.1051/analusis:1999108

Development of an in vitro test for screening of chelators of uranium

O. Braun1, C. Contino1, M.-H. Hengé-Napoli2, E. Ansoborlo2 and B. Pucci1

1  Laboratoire de Chimie Bioorganique et des Systémes Moléculaires Vectoriels, Faculté des Sciences d'Avignon, 33 rue Louis Pasteur, 84000-Avignon, France
2  Institut de Protection et de Sûreté Nucléaire, Département de Protection de la Santé de l'Homme et de Dosimétrie, Service de Dosimétrie, BP. 6, 92265 Fontenay-aux-Roses Cedex, France

This work deals with an in vitro spectrophotometric method to evaluate the chelating ability of various organic ligands for uranyl ion. In this way, an uranium complex formed with a peculiar ligand is chosen as a reference. Any modification of the UV-Visible absorbance of the U(VI) reference complex, owing to its dissociation upon the addition of a new ligand, permits to compare the affinity of the latter ligand for U(VI) with that of the reference ligand. This test allows to screen easily a lot of ligands before to evaluate their in vivo uranium chelating property.

Key words: Uranium (VI) titrimetry / uranium ligands / Chromotrope 2R complexes.

© EDP Sciences, Wiley-VCH 1999