Volume 26, Number 10, December 1998MALDI-TOF
|Page(s)||396 - 399|
Comparison of High-Performance Ion Chromatography and absorptiometric methods for the determination of phytic acid in food samplesP. Talamond1, G. Gallon2, J.P. Guyot1, I. Mbome Lape3 and S. Trèche2
1 Laboratoire de Biotechnologie Microbienne Tropicale, Centre ORSTOM de Montpellier, BP. 5045, 911 avenue d'Agropolis, 34032 Montpellier Cedex, France
2 Laboratoire de Nutrition Tropicale, Centre ORSTOM de Montpellier, BP. 5045, 911 avenue d'Agropolis, 34032 Montpellier Cedex, France
3 Food and Nutrition Research Centre, P.O. Box 6163, Yaoundé, Cameroon
The objective of this paper consists in defining the interest of a new high-performance ion chromatography method (HPIC) with chemically suppressed conductivity detector for phytic acid determination in food samples. Firsly, accuracy and precision of the HPIC method have been measured. Secondly, The HPIC method and a classical absorptiometric method were compared. The HPIC method was more sensitive and selective than the absorptiometric method which leaded to an 27% overestimation of the phytic acid content in legume seeds. Because of it is rapid and easy to perform, the HPIC method appears to be particularly suitable for routine analysis of food samples.
Key words: Phytic acid / liquid chromatography / absorptiometry / food analysis.
© EDP Sciences, Wiley-VCH 1998