Issue |
Analusis
Volume 28, Number 6, July/August 2000
Nitric oxide and superoxide in biological systems
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Page(s) | 543 - 549 | |
Section | Original articles | |
DOI | https://doi.org/10.1051/analusis:2000169 |
DOI: 10.1051/analusis:2000169
Influence of secondary interactions on high performance size exclusion chromatography. Application to the fractionation of landfill leachates
F. Le Coupannec, D. Morin, O. Sire and J.-J. PéronLaboratoire de Biologie et de Chimie Moléculaires, Université de Bretagne Sud, rue St-Maudé, 56325 Lorient, France
(Received March 30, 2000; revised July 17, 2000; accepted July 19, 2000.)
Abstract
The leachates from municipal landfills are characterised by the complex chemical
distribution and wide molecular weights range of their constituents. We have
designed a high performance size exclusion chromatography method to take into
account these two aspects. By using eluents of low ionic strength, electrostatic
interactions between the eluted molecules and the negative sites of the stationary
phase are enhanced. Thus, the compounds are eluted mostly according to their
physico-chemical properties. Contrarily, using eluents of high ionic strength allows
eliminating most of these electrostatic interactions which, in turn, permits to
estimate the molecular weights, since steric exclusion is then the dominating mechanism
of separation. A spectroscopic fingerprint may also be determined through multidetection
(UV absorption, fluorescence and evaporative light scattering detection) coupled on line
which allows to sort out the compounds into a few number of families.
Key words: Landfill leachates -- high performance size exclusion chromatography -- secondary interactions -- characterisation.
© EDP Sciences, Wiley-VCH 2000