Free Access
Volume 28, Number 6, July/August 2000
Nitric oxide and superoxide in biological systems
Page(s) 529 - 534
Section Original articles
Analusis 28, 529-534 (2000)
DOI: 10.1051/analusis:2000168

Use of a chloride ion selective electrode as a tool for monitoring relatively fast kinetic processes. Application to the hydrolysis of yperite analogues in presence of oximate nucleophiles

S. Pereira Rodrigues1, L. Coppée2, J. Toullec1 and G. Moutiers1

1  SIRCOB, ESA 8086 CNRS, Bâtiment Lavoisier, Université de Versailles, 45, avenue des États-Unis, 78035 Versailles Cedex, France
2  Centre d'Études du Bouchet, BP 3, 91719 Vert-le-Petit, France

(Received March 10, 2000; accepted April 27, 2000.)

Experimental conditions have been designed which allowed the use of a chloride ion selective electrode for the monitoring of reactions that proceed with a moderately fast departure of Cl- ion, i.e. t $_{1/2}\,\,\approx$ 40 seconds, in aqueous solution. The procedure has been applied in particular to the hydrolysis of yperite (HD) analogues (CEES, CEMS, CEPS) in aqueous solution in presence of HEPES buffer and oximate nucleophiles. Kinetic parameters were obtained in these conditions and the S$_{\rm N}$1 mechanism for the hydrolysis was confirmed.

Key words: Chloride ion selective electrode -- oximate ion -- reactivity -- kinetics -- yperite -- yperite analogues -- yperite hydrolysis.

© EDP Sciences, Wiley-VCH 2000