Volume 27, Number 5, June 1999Humic substances
|Page(s)||447 - 452|
Purification of 2-methoxyethyl 1-methylethyl 2, 6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate by preparative high-performance liquid chromatographyI. Medina
Departamento de Ingeniería Química, Universidad de Oviedo, 33071 Oviedo, Spain
The 2-methoxyethyl 1-methylethyl 2,6-dimethyl-4-(3-nitrophenyl)pyridine-3, 5-dicarboxylate was obtained in a 99.8% pure form from raw material by preparative-scale high performance liquid chromatography. This compound is an impurity in the industrial process of nimodipine. The chromatographic conditions were optimized to achieve any efficient purification in the shortest time. A reversed-phase preparative-scale HPLC column was used. This work demonstrates how gram quantities of pure material can be conveniently obtained using preparative HPLC, even when the components in a mixture are difficult to resolve.
Key words: Calcium antagonist / nimodipine / column liquid chromatography / preparative scale separation.
© EDP Sciences, Wiley-VCH 1999