Free Access
Issue
Analusis
Volume 27, Number 5, June 1999
Humic substances
Page(s) 447 - 452
Section Original articles
DOI https://doi.org/10.1051/analusis:1999123
Analusis 27, 447-452 (1999)
DOI: 10.1051/analusis:1999123

Purification of 2-methoxyethyl 1-methylethyl 2, 6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate by preparative high-performance liquid chromatography

I. Medina

Departamento de Ingeniería Química, Universidad de Oviedo, 33071 Oviedo, Spain


Abstract
The 2-methoxyethyl 1-methylethyl 2,6-dimethyl-4-(3-nitrophenyl)pyridine-3, 5-dicarboxylate was obtained in a 99.8% pure form from raw material by preparative-scale high performance liquid chromatography. This compound is an impurity in the industrial process of nimodipine. The chromatographic conditions were optimized to achieve any efficient purification in the shortest time. A reversed-phase preparative-scale HPLC column was used. This work demonstrates how gram quantities of pure material can be conveniently obtained using preparative HPLC, even when the components in a mixture are difficult to resolve.


Key words: Calcium antagonist / nimodipine / column liquid chromatography / preparative scale separation.


© EDP Sciences, Wiley-VCH 1999

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.