Analusis 27, 284-288 (1999)
DOI: 10.1051/analusis:1999118

Separation and quantitation of orange juices using liquid chromatography of polymethoxylated flavones

P.P. Mouly¹, E.M. Gaydou² and C. Arzouyan<sup>1

1</sup>  SGS-RVAC (Société Générale de Surveillance-CEntre de Recherche et de VAlorisation des produits de la Consommation, Parc Club des Aygalades, Bât. A7, Z.I.N. 1207, 35 Boulevard du Capitaine Gèze, 13333 Marseille Cedex 14, France
²  Laboratoire de Phytochimie de Marseille, Faculté des Sciences et Techniques de Saint Jérôme, Avenue Escadrille Normandie Niemen, 13397 Marseille Cedex 13, France

Abstract
A rapid method of extraction and quantitation of polymethoxylated flavones (PMF) using isocratic liquid chromatography is described. With this method the content of six PMF: sinensetin, hexamethoxyflavone, nobiletin, heptamethoxyflavone, tetra-O-methyl scutellarein and tangeretin from 60 commercial orange juices have been determined. The PMF extraction method is mainly established on sample preparations using C₁₈ cartridge extractions with water/acetonitrile (60/40, v/v) as mobile phase. PMF compositions of orange commercial juices show a quantitative difference on population samples which are linked to the PMF contents and varieties. By these rapid quantitative method, the PMF values observed were in accordance with literature. This technique present correct characteristics of repeatability and reproducibility (4.5% and 6.6% respectively).

Key words: Polymethoxylated flavones / extraction / determinations / liquid chromatography / orange juice.


© EDP Sciences, Wiley-VCH 1999