Issue |
Analusis
Volume 26, Number 7, September 1998
Preparative Chromatography
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Page(s) | 231 - 236 | |
Section | Original articles | |
DOI | https://doi.org/10.1051/analusis:1998166 |
DOI: 10.1051/analusis:1998166
Theory of bulk and flow electrolysis and approach to parameter optimisation for chromatographic electrochemical detection
O. Abollino, M.C. Bruzzoniti, E. Mentasti and C. SarzaniniDepartment of Analytical Chemistry, University of Torino, Via P. Giuria 5, 10125 Torino, Italy
Abstract
The performance and characteristics of an amperometric-coulometric detector
for liquid chromatography were investigated as a function of the main
chromatographic parameters. Hydroquinone was chosen as reference compound.
Starting from the theory of bulk and flow electrolysis, the effect of eluent
flow rate on the detector response was investigated and the agreement between
experimental and calculated data was verified. The study was preceded by an
investigation of the detector behaviour in different chromatographic operating
conditions: void volume evaluation, effect of mobile phase composition on
baseline behaviour, current/potential curve for analyte and mobile phase,
analyte stability, linearity, repeatability of the detector response and
electrochemical exclusion from interferences were examined.
Key words: Electrochemical detection / liquid chromatography / electrolysis / hydroquinon -- catechol.
© EDP Sciences, Wiley-VCH 1998