Free Access
| Issue |
Analusis
Volume 26, Number 5, June 1998
Microcolumn separation techniques developing in Japan
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|---|---|---|
| Page(s) | 219 - 222 | |
| Section | Original articles | |
| DOI | https://doi.org/10.1051/analusis:1998138 | |
Analusis 26, 219-222 (1998)
DOI: 10.1051/analusis:1998138
1 Laboratorio di Biochimica Clinica, Ospedale Pediatrico "IRCCS Bambino Gesù", Piazzale S. Onofrio n° 4, 00165 Rome, Italy
2 Dipartimento di Scienze e Tecnologie Chimiche, Universitá Tor Vergata, via della Ricerca Scientifica, 00133 Rome, Italy
© EDP Sciences, Wiley-VCH 1998
DOI: 10.1051/analusis:1998138
Electrochemical probe for polyamines detection in biological fluids
L. Scarciglia1, D. Compagnone2, G. Federici1 and G. Palleschi21 Laboratorio di Biochimica Clinica, Ospedale Pediatrico "IRCCS Bambino Gesù", Piazzale S. Onofrio n° 4, 00165 Rome, Italy
2 Dipartimento di Scienze e Tecnologie Chimiche, Universitá Tor Vergata, via della Ricerca Scientifica, 00133 Rome, Italy
Abstract
The enzyme polyamine oxidase from maize has been purified and covalently immobilized onto polymeric supports to assemble a polyamine electrochemical biosensor. The enzyme has been used
in conjunction with either an O2 or a H2O2 electrode. The analytical behaviour of this enzyme electrode has been studied with respect to sensitivity, selectivity, optimum pH and buffer, response
time and lifetime. Recovery studies performed on urine samples demonstrated that the polyamine biosensor developed can be successfully used to measure polyamines in biological fluids.
© EDP Sciences, Wiley-VCH 1998