Electrochemical probe for polyamines detection in biological fluidsL. Scarciglia1, D. Compagnone2, G. Federici1 and G. Palleschi2
1 Laboratorio di Biochimica Clinica, Ospedale Pediatrico "IRCCS Bambino Gesù", Piazzale S. Onofrio n° 4, 00165 Rome, Italy
2 Dipartimento di Scienze e Tecnologie Chimiche, Universitá Tor Vergata, via della Ricerca Scientifica, 00133 Rome, Italy
The enzyme polyamine oxidase from maize has been purified and covalently immobilized onto polymeric supports to assemble a polyamine electrochemical biosensor. The enzyme has been used in conjunction with either an O2 or a H2O2 electrode. The analytical behaviour of this enzyme electrode has been studied with respect to sensitivity, selectivity, optimum pH and buffer, response time and lifetime. Recovery studies performed on urine samples demonstrated that the polyamine biosensor developed can be successfully used to measure polyamines in biological fluids.
© EDP Sciences, Wiley-VCH 1998