Detection of the radiolysis of solid ampicillin by UV - spectroscopyM. Gibella and B. Tilquin
Université catholique de Louvain, Unité d'Analyse Chimique et Physico-Chimique des Médicaments, Laboratoire de Radiostérilisation, Pharmacie, UCL-7230, avenue Mounier 72, 1200 Brussels, Belgium
(Received January, 22, 1999; revised July, 7, 1999; accepted July, 15, 1999.)
We propose to detect the use of g-rays in order to radiosterilize ampicillin by UV absorption spectroscopy. The UV absorption results are verified by comparison with HPLC-diode array analysis. The sensitivity is excellent (10-6 M). The wavelengths in the range from 287 to 335 nm are characteristics of radiolysis related substances. It is possible to use this range of wavelengths to detect the radiolysis of ampicillin with an usual UV spectrophotometer.
Key words: antibiotic / radiosterilization / capillary liquid-liquid chromatography / UV-detection.
© EDP Sciences, Wiley-VCH 1999